Construct: shRNA TRCN0000241797

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001045807.1-2877s21c1
DNA Barcode:
AGGAACCTTGTGTCCTATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Rbm15 (229700)
Gene Description:
RNA binding motif protein 15
Transcript:
RefSeq NM_001045807.1 (CURRENT)
Match location:
Position 2877 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241797)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGAACCTTGTGTCCTATTTA
Hairpin Sequence:
5'-CCGG-AGGAACCTTGTGTCCTATTTA-CTCGAG-TAAATAGGACACAAGGTTCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGAACCTTGTGTCCTATTTACTCGAGTAAATAGGACACAAGGTTCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGAACCTTGTGTCCTATTTACTCGAGTAAATAGGACACAAGGTTCCT-3'

Other clones with same target sequence:

  1. TRCN0000233468