Construct: shRNA TRCN0000241838

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032334.1-741s21c1
DNA Barcode:
GCACCGGACACACAATCATCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UTP23 (84294)
Gene Description:
UTP23 small subunit processome component
Transcript:
RefSeq NM_032334.1 (NON-CURRENT)
Match location:
Position 741 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241838)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCACCGGACACACAATCATCT
Hairpin Sequence:
5'-CCGG-GCACCGGACACACAATCATCT-CTCGAG-AGATGATTGTGTGTCCGGTGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCACCGGACACACAATCATCTCTCGAGAGATGATTGTGTGTCCGGTGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCACCGGACACACAATCATCTCTCGAGAGATGATTGTGTGTCCGGTGC-3'

Other clones with same target sequence:

(none)