Construct: shRNA TRCN0000241860

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_032884.3-1755s21c1
DNA Barcode:
CAAGACCAGATTCATCTATTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
C1orf94 (84970)
Gene Description:
chromosome 1 open reading frame 94
Transcript:
RefSeq NM_032884.3 (NON-CURRENT)
Match location:
Position 1755 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241860)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAAGACCAGATTCATCTATTG
Hairpin Sequence:
5'-CCGG-CAAGACCAGATTCATCTATTG-CTCGAG-CAATAGATGAATCTGGTCTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAAGACCAGATTCATCTATTGCTCGAGCAATAGATGAATCTGGTCTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAAGACCAGATTCATCTATTGCTCGAGCAATAGATGAATCTGGTCTTG-3'

Other clones with same target sequence:

(none)