Construct: shRNA TRCN0000241868

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_139320.1-907s21c1
DNA Barcode:
AGAGGAGTGAAAGGTTCTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CHRFAM7A (89832)
Gene Description:
CHRNA7 (exons 5-10) and FAM7A (exons A-E) fusion
Transcript:
RefSeq NM_139320.1 (CURRENT)
Match location:
Position 907 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241868)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAGGAGTGAAAGGTTCTATG
Hairpin Sequence:
5'-CCGG-AGAGGAGTGAAAGGTTCTATG-CTCGAG-CATAGAACCTTTCACTCCTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAGGAGTGAAAGGTTCTATGCTCGAGCATAGAACCTTTCACTCCTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAGGAGTGAAAGGTTCTATGCTCGAGCATAGAACCTTTCACTCCTCT-3'

Other clones with same target sequence:

(none)