Construct: shRNA TRCN0000241874

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039841.1-722s21c1
DNA Barcode:
AGCAATCTTGCAGTAATATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ARHGAP11B (89839)
Gene Description:
Rho GTPase activating protein 11B
Transcript:
RefSeq NM_001039841.1 (NON-CURRENT)
Match location:
Position 722 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241874)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGCAATCTTGCAGTAATATTT
Hairpin Sequence:
5'-CCGG-AGCAATCTTGCAGTAATATTT-CTCGAG-AAATATTACTGCAAGATTGCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGCAATCTTGCAGTAATATTTCTCGAGAAATATTACTGCAAGATTGCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGCAATCTTGCAGTAATATTTCTCGAGAAATATTACTGCAAGATTGCT-3'

Other clones with same target sequence:

(none)