Construct: shRNA TRCN0000241896

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_153516.2-564s21c1
DNA Barcode:
ATATGAGGCATATCGTGAATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bcl2l13 (94044)
Gene Description:
BCL2-like 13 (apoptosis facilitator)
Transcript:
RefSeq NM_153516.2 (CURRENT)
Match location:
Position 564 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241896)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATATGAGGCATATCGTGAATG
Hairpin Sequence:
5'-CCGG-ATATGAGGCATATCGTGAATG-CTCGAG-CATTCACGATATGCCTCATAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATATGAGGCATATCGTGAATGCTCGAGCATTCACGATATGCCTCATATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATATGAGGCATATCGTGAATGCTCGAGCATTCACGATATGCCTCATAT-3'

Other clones with same target sequence:

(none)