Construct: shRNA TRCN0000241905

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_133798.2-727s21c1
DNA Barcode:
ACAAGCTTCTACAGGATTATG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Exd2 (97827)
Gene Description:
exonuclease 3'-5' domain containing 2
Transcript:
RefSeq NM_133798.2 (NON-CURRENT)
Match location:
Position 727 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241905)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAAGCTTCTACAGGATTATG
Hairpin Sequence:
5'-CCGG-ACAAGCTTCTACAGGATTATG-CTCGAG-CATAATCCTGTAGAAGCTTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAAGCTTCTACAGGATTATGCTCGAGCATAATCCTGTAGAAGCTTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAAGCTTCTACAGGATTATGCTCGAGCATAATCCTGTAGAAGCTTGT-3'

Other clones with same target sequence:

(none)