Construct: shRNA TRCN0000241978

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033217.4-3379s21c1
DNA Barcode:
TTACACTGCCATGCTAGATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Prickle1 (106042)
Gene Description:
prickle planar cell polarity protein 1
Transcript:
RefSeq NM_001033217.4 (CURRENT)
Match location:
Position 3379 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000241978)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TTACACTGCCATGCTAGATTT
Hairpin Sequence:
5'-CCGG-TTACACTGCCATGCTAGATTT-CTCGAG-AAATCTAGCATGGCAGTGTAA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTTACACTGCCATGCTAGATTTCTCGAGAAATCTAGCATGGCAGTGTAATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATTACACTGCCATGCTAGATTTCTCGAGAAATCTAGCATGGCAGTGTAA-3'

Other clones with same target sequence:

(none)