Construct: shRNA TRCN0000242003

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_179203.2-2231s21c1
DNA Barcode:
GGACATTACTCCTACCATATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Atad3a (108888)
Gene Description:
ATPase family, AAA domain containing 3A
Transcript:
RefSeq NM_179203.2 (NON-CURRENT)
Match location:
Position 2231 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242003)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGACATTACTCCTACCATATA
Hairpin Sequence:
5'-CCGG-GGACATTACTCCTACCATATA-CTCGAG-TATATGGTAGGAGTAATGTCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGACATTACTCCTACCATATACTCGAGTATATGGTAGGAGTAATGTCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGACATTACTCCTACCATATACTCGAGTATATGGTAGGAGTAATGTCC-3'

Other clones with same target sequence:

(none)