Construct: shRNA TRCN0000242045

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_001473991.1-6447s21c1
DNA Barcode:
AGTTCCTGGACCACGAGATTG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Shank1 (243961)
Gene Description:
SH3/ankyrin domain gene 1
Transcript:
RefSeq XM_001473991.1 (NON-CURRENT)
Match location:
Position 6447 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242045)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGTTCCTGGACCACGAGATTG
Hairpin Sequence:
5'-CCGG-AGTTCCTGGACCACGAGATTG-CTCGAG-CAATCTCGTGGTCCAGGAACT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGTTCCTGGACCACGAGATTGCTCGAGCAATCTCGTGGTCCAGGAACTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGTTCCTGGACCACGAGATTGCTCGAGCAATCTCGTGGTCCAGGAACT-3'

Other clones with same target sequence:

(none)