Construct: shRNA TRCN0000242049

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_173189.2-2602s21c1
DNA Barcode:
TGCCGACTTGAACGCCATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Mcph1 (244329)
Gene Description:
microcephaly, primary autosomal recessive 1
Transcript:
RefSeq NM_173189.2 (CURRENT)
Match location:
Position 2602 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242049)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCCGACTTGAACGCCATTTA
Hairpin Sequence:
5'-CCGG-TGCCGACTTGAACGCCATTTA-CTCGAG-TAAATGGCGTTCAAGTCGGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCCGACTTGAACGCCATTTACTCGAGTAAATGGCGTTCAAGTCGGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCCGACTTGAACGCCATTTACTCGAGTAAATGGCGTTCAAGTCGGCA-3'

Other clones with same target sequence:

(none)