Construct: shRNA TRCN0000242054

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_905108.3-1777s21c1
DNA Barcode:
AGGATAGAGCTTACTCCTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cntn5 (244682)
Gene Description:
contactin 5
Transcript:
RefSeq XM_905108.3 (NON-CURRENT)
Match location:
Position 1777 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242054)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGGATAGAGCTTACTCCTAAA
Hairpin Sequence:
5'-CCGG-AGGATAGAGCTTACTCCTAAA-CTCGAG-TTTAGGAGTAAGCTCTATCCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGGATAGAGCTTACTCCTAAACTCGAGTTTAGGAGTAAGCTCTATCCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGGATAGAGCTTACTCCTAAACTCGAGTTTAGGAGTAAGCTCTATCCT-3'

Other clones with same target sequence:

(none)