Construct: shRNA TRCN0000242074

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_198423.3-8969s21c1
DNA Barcode:
GAAATTACACTGCGGTTTATT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Bahcc1 (268515)
Gene Description:
BAH domain and coiled-coil containing 1
Transcript:
RefSeq NM_198423.3 (CURRENT)
Match location:
Position 8969 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242074)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAAATTACACTGCGGTTTATT
Hairpin Sequence:
5'-CCGG-GAAATTACACTGCGGTTTATT-CTCGAG-AATAAACCGCAGTGTAATTTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAAATTACACTGCGGTTTATTCTCGAGAATAAACCGCAGTGTAATTTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAAATTACACTGCGGTTTATTCTCGAGAATAAACCGCAGTGTAATTTC-3'

Other clones with same target sequence:

(none)