Construct: shRNA TRCN0000242102

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_178760.3-1000s21c1
DNA Barcode:
TGAAGGCTGGGCTGTTGTATA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpr107 (277463)
Gene Description:
G protein-coupled receptor 107
Transcript:
RefSeq NM_178760.3 (NON-CURRENT)
Match location:
Position 1000 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242102)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGAAGGCTGGGCTGTTGTATA
Hairpin Sequence:
5'-CCGG-TGAAGGCTGGGCTGTTGTATA-CTCGAG-TATACAACAGCCCAGCCTTCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGAAGGCTGGGCTGTTGTATACTCGAGTATACAACAGCCCAGCCTTCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGAAGGCTGGGCTGTTGTATACTCGAGTATACAACAGCCCAGCCTTCA-3'

Other clones with same target sequence:

(none)