Construct: shRNA TRCN0000242154

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001033389.2-857s21c1
DNA Barcode:
CATAGGAAGGAACGGAGAAAT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Fubp3 (320267)
Gene Description:
far upstream element (FUSE) binding protein 3
Transcript:
RefSeq NM_001033389.2 (NON-CURRENT)
Match location:
Position 857 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242154)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATAGGAAGGAACGGAGAAAT
Hairpin Sequence:
5'-CCGG-CATAGGAAGGAACGGAGAAAT-CTCGAG-ATTTCTCCGTTCCTTCCTATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATAGGAAGGAACGGAGAAATCTCGAGATTTCTCCGTTCCTTCCTATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATAGGAAGGAACGGAGAAATCTCGAGATTTCTCCGTTCCTTCCTATG-3'

Other clones with same target sequence:

(none)