Construct: shRNA TRCN0000242177

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_207255.1-2928s21c1
DNA Barcode:
GATCAAGGACCCTGATGTAAA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp532 (328977)
Gene Description:
zinc finger protein 532
Transcript:
RefSeq NM_207255.1 (NON-CURRENT)
Match location:
Position 2928 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242177)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GATCAAGGACCCTGATGTAAA
Hairpin Sequence:
5'-CCGG-GATCAAGGACCCTGATGTAAA-CTCGAG-TTTACATCAGGGTCCTTGATC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGATCAAGGACCCTGATGTAAACTCGAGTTTACATCAGGGTCCTTGATCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGATCAAGGACCCTGATGTAAACTCGAGTTTACATCAGGGTCCTTGATC-3'

Other clones with same target sequence:

(none)