Construct: shRNA TRCN0000242183

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_183033.1-3191s21c1
DNA Barcode:
TGGATATCCTCAGTATCTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Zfp516 (329003)
Gene Description:
zinc finger protein 516
Transcript:
RefSeq NM_183033.1 (NON-CURRENT)
Match location:
Position 3191 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242183)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGATATCCTCAGTATCTTTA
Hairpin Sequence:
5'-CCGG-TGGATATCCTCAGTATCTTTA-CTCGAG-TAAAGATACTGAGGATATCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGATATCCTCAGTATCTTTACTCGAGTAAAGATACTGAGGATATCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGATATCCTCAGTATCTTTACTCGAGTAAAGATACTGAGGATATCCA-3'

Other clones with same target sequence:

(none)