Construct: shRNA TRCN0000242243

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001001415.2-2027s21c1
DNA Barcode:
TACCCGGTCTTCAAGACTTAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ZNF429 (353088)
Gene Description:
zinc finger protein 429
Transcript:
RefSeq NM_001001415.2 (NON-CURRENT)
Match location:
Position 2027 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242243)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TACCCGGTCTTCAAGACTTAC
Hairpin Sequence:
5'-CCGG-TACCCGGTCTTCAAGACTTAC-CTCGAG-GTAAGTCTTGAAGACCGGGTA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTACCCGGTCTTCAAGACTTACCTCGAGGTAAGTCTTGAAGACCGGGTATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATACCCGGTCTTCAAGACTTACCTCGAGGTAAGTCTTGAAGACCGGGTA-3'

Other clones with same target sequence:

(none)