Construct: shRNA TRCN0000242282

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001004062.2-539s21c1
DNA Barcode:
CGCACCAGAAGAGAGTCTTAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Crtc1 (382056)
Gene Description:
CREB regulated transcription coactivator 1
Transcript:
RefSeq NM_001004062.2 (CURRENT)
Match location:
Position 539 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242282)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCACCAGAAGAGAGTCTTAC
Hairpin Sequence:
5'-CCGG-CGCACCAGAAGAGAGTCTTAC-CTCGAG-GTAAGACTCTCTTCTGGTGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCACCAGAAGAGAGTCTTACCTCGAGGTAAGACTCTCTTCTGGTGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCACCAGAAGAGAGTCTTACCTCGAGGTAAGACTCTCTTCTGGTGCG-3'

Other clones with same target sequence:

(none)