Construct: shRNA TRCN0000242319

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001013660.2-1069s21c1
DNA Barcode:
AGATTCCTGGTCCTATAATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FRRS1 (391059)
Gene Description:
ferric chelate reductase 1
Transcript:
RefSeq NM_001013660.2 (NON-CURRENT)
Match location:
Position 1069 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242319)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGATTCCTGGTCCTATAATTT
Hairpin Sequence:
5'-CCGG-AGATTCCTGGTCCTATAATTT-CTCGAG-AAATTATAGGACCAGGAATCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGATTCCTGGTCCTATAATTTCTCGAGAAATTATAGGACCAGGAATCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGATTCCTGGTCCTATAATTTCTCGAGAAATTATAGGACCAGGAATCT-3'

Other clones with same target sequence:

(none)