Construct: shRNA TRCN0000242401

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001101804.1-977s21c1
DNA Barcode:
ATAGCAGTCATCTCCACAAAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gm14434 (668039)
Gene Description:
predicted gene 14434
Transcript:
RefSeq NM_001101804.1 (CURRENT)
Match location:
Position 977 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242401)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATAGCAGTCATCTCCACAAAC
Hairpin Sequence:
5'-CCGG-ATAGCAGTCATCTCCACAAAC-CTCGAG-GTTTGTGGAGATGACTGCTAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATAGCAGTCATCTCCACAAACCTCGAGGTTTGTGGAGATGACTGCTATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATAGCAGTCATCTCCACAAACCTCGAGGTTTGTGGAGATGACTGCTAT-3'

Other clones with same target sequence:

(none)