Construct: shRNA TRCN0000242480

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_014044.5-467s21c1
DNA Barcode:
GGGATTCTTTGAGACAATAAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
UNC50 (25972)
Gene Description:
unc-50 inner nuclear membrane RNA binding protein
Transcript:
RefSeq NM_014044.5 (NON-CURRENT)
Match location:
Position 467 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242480)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GGGATTCTTTGAGACAATAAA
Hairpin Sequence:
5'-CCGG-GGGATTCTTTGAGACAATAAA-CTCGAG-TTTATTGTCTCAAAGAATCCC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGGGATTCTTTGAGACAATAAACTCGAGTTTATTGTCTCAAAGAATCCCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGGGATTCTTTGAGACAATAAACTCGAGTTTATTGTCTCAAAGAATCCC-3'

Other clones with same target sequence:

(none)