Construct: shRNA TRCN0000242509

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018491.3-239s21c1
DNA Barcode:
ACAATTATCACCGGGTATTTA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CBWD1 (55871)
Gene Description:
COBW domain containing 1
Transcript:
RefSeq NM_018491.3 (CURRENT)
Match location:
Position 239 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242509)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACAATTATCACCGGGTATTTA
Hairpin Sequence:
5'-CCGG-ACAATTATCACCGGGTATTTA-CTCGAG-TAAATACCCGGTGATAATTGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACAATTATCACCGGGTATTTACTCGAGTAAATACCCGGTGATAATTGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACAATTATCACCGGGTATTTACTCGAGTAAATACCCGGTGATAATTGT-3'

Other clones with same target sequence:

(none)