Construct: shRNA TRCN0000242550

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182762.3-969s21c1
DNA Barcode:
TCAAGGAGGGTCAGTACAATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MACC1 (346389)
Gene Description:
MET transcriptional regulator MACC1
Transcript:
RefSeq NM_182762.3 (NON-CURRENT)
Match location:
Position 969 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242550)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCAAGGAGGGTCAGTACAATT
Hairpin Sequence:
5'-CCGG-TCAAGGAGGGTCAGTACAATT-CTCGAG-AATTGTACTGACCCTCCTTGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCAAGGAGGGTCAGTACAATTCTCGAGAATTGTACTGACCCTCCTTGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCAAGGAGGGTCAGTACAATTCTCGAGAATTGTACTGACCCTCCTTGA-3'

Other clones with same target sequence:

(none)