Construct: shRNA TRCN0000242606

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001501.1-105s21c1
DNA Barcode:
TGGACCCTCAGAGGCTCAGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GNRH2 (2797)
Gene Description:
gonadotropin releasing hormone 2
Transcript:
RefSeq NM_001501.1 (NON-CURRENT)
Match location:
Position 105 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242606)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGACCCTCAGAGGCTCAGCA
Hairpin Sequence:
5'-CCGG-TGGACCCTCAGAGGCTCAGCA-CTCGAG-TGCTGAGCCTCTGAGGGTCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGACCCTCAGAGGCTCAGCACTCGAGTGCTGAGCCTCTGAGGGTCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGACCCTCAGAGGCTCAGCACTCGAGTGCTGAGCCTCTGAGGGTCCA-3'

Other clones with same target sequence:

(none)