Construct: shRNA TRCN0000242650

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_080626.5-461s21c1
DNA Barcode:
CTCCAACCTGTCCCAGTATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BRI3BP (140707)
Gene Description:
BRI3 binding protein
Transcript:
RefSeq NM_080626.5 (NON-CURRENT)
Match location:
Position 461 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242650)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTCCAACCTGTCCCAGTATTT
Hairpin Sequence:
5'-CCGG-CTCCAACCTGTCCCAGTATTT-CTCGAG-AAATACTGGGACAGGTTGGAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTCCAACCTGTCCCAGTATTTCTCGAGAAATACTGGGACAGGTTGGAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTCCAACCTGTCCCAGTATTTCTCGAGAAATACTGGGACAGGTTGGAG-3'

Other clones with same target sequence:

(none)