Construct: shRNA TRCN0000242694

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001109763.1-936s21c1
DNA Barcode:
GCGAGGAGGACTTTCACTTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GSG1L (146395)
Gene Description:
GSG1 like
Transcript:
RefSeq NM_001109763.1 (NON-CURRENT)
Match location:
Position 936 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242694)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GCGAGGAGGACTTTCACTTAG
Hairpin Sequence:
5'-CCGG-GCGAGGAGGACTTTCACTTAG-CTCGAG-CTAAGTGAAAGTCCTCCTCGC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGCGAGGAGGACTTTCACTTAGCTCGAGCTAAGTGAAAGTCCTCCTCGCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGCGAGGAGGACTTTCACTTAGCTCGAGCTAAGTGAAAGTCCTCCTCGC-3'

Other clones with same target sequence:

(none)