Construct: shRNA TRCN0000242720

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001141972.1-387s21c1
DNA Barcode:
ATGACCTTGCTTGCTGAATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DPH6 (89978)
Gene Description:
diphthamine biosynthesis 6
Transcript:
RefSeq NM_001141972.1 (NON-CURRENT)
Match location:
Position 387 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242720)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGACCTTGCTTGCTGAATAT
Hairpin Sequence:
5'-CCGG-ATGACCTTGCTTGCTGAATAT-CTCGAG-ATATTCAGCAAGCAAGGTCAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGACCTTGCTTGCTGAATATCTCGAGATATTCAGCAAGCAAGGTCATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGACCTTGCTTGCTGAATATCTCGAGATATTCAGCAAGCAAGGTCAT-3'

Other clones with same target sequence:

(none)