Construct: shRNA TRCN0000242736

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001475.1-135s21c1
DNA Barcode:
CCTGAAGTGATTGGGCCTATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
GAGE5 (2577)
Gene Description:
G antigen 5
Transcript:
RefSeq NM_001475.1 (CURRENT)
Match location:
Position 135 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242736)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTGAAGTGATTGGGCCTATG
Hairpin Sequence:
5'-CCGG-CCTGAAGTGATTGGGCCTATG-CTCGAG-CATAGGCCCAATCACTTCAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTGAAGTGATTGGGCCTATGCTCGAGCATAGGCCCAATCACTTCAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTGAAGTGATTGGGCCTATGCTCGAGCATAGGCCCAATCACTTCAGG-3'

Other clones with same target sequence:

(none)