Construct: shRNA TRCN0000242766

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_018469.3-4068s21c1
DNA Barcode:
GAGATGGGCAGAGCAACTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
TEX2 (55852)
Gene Description:
testis expressed 2
Transcript:
RefSeq NM_018469.3 (NON-CURRENT)
Match location:
Position 4068 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242766)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
GAGATGGGCAGAGCAACTTAT
Hairpin Sequence:
5'-CCGG-GAGATGGGCAGAGCAACTTAT-CTCGAG-ATAAGTTGCTCTGCCCATCTC-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGGAGATGGGCAGAGCAACTTATCTCGAGATAAGTTGCTCTGCCCATCTCTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAGAGATGGGCAGAGCAACTTATCTCGAGATAAGTTGCTCTGCCCATCTC-3'

Other clones with same target sequence:

(none)