Construct: shRNA TRCN0000242779

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001131067.1-1644s21c1
DNA Barcode:
CATGACCAAGGCGTTGCAGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NKPD1 (284353)
Gene Description:
NTPase KAP family P-loop domain containing 1
Transcript:
RefSeq NM_001131067.1 (NON-CURRENT)
Match location:
Position 1644 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242779)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATGACCAAGGCGTTGCAGAA
Hairpin Sequence:
5'-CCGG-CATGACCAAGGCGTTGCAGAA-CTCGAG-TTCTGCAACGCCTTGGTCATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATGACCAAGGCGTTGCAGAACTCGAGTTCTGCAACGCCTTGGTCATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATGACCAAGGCGTTGCAGAACTCGAGTTCTGCAACGCCTTGGTCATG-3'

Other clones with same target sequence:

(none)