Construct: shRNA TRCN0000242882

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001002017.1-447s21c1
DNA Barcode:
TCTGCGCAAGCAGTTTCTGTC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
HCFC1R1 (54985)
Gene Description:
host cell factor C1 regulator 1
Transcript:
RefSeq NM_001002017.1 (NON-CURRENT)
Match location:
Position 447 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242882)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TCTGCGCAAGCAGTTTCTGTC
Hairpin Sequence:
5'-CCGG-TCTGCGCAAGCAGTTTCTGTC-CTCGAG-GACAGAAACTGCTTGCGCAGA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTCTGCGCAAGCAGTTTCTGTCCTCGAGGACAGAAACTGCTTGCGCAGATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATCTGCGCAAGCAGTTTCTGTCCTCGAGGACAGAAACTGCTTGCGCAGA-3'

Other clones with same target sequence:

(none)