Construct: shRNA TRCN0000242898

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152499.1-1290s21c1
DNA Barcode:
CATCGGTCCCAAGCATCAAAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CCDC24 (149473)
Gene Description:
coiled-coil domain containing 24
Transcript:
RefSeq NM_152499.1 (NON-CURRENT)
Match location:
Position 1290 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242898)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CATCGGTCCCAAGCATCAAAG
Hairpin Sequence:
5'-CCGG-CATCGGTCCCAAGCATCAAAG-CTCGAG-CTTTGATGCTTGGGACCGATG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCATCGGTCCCAAGCATCAAAGCTCGAGCTTTGATGCTTGGGACCGATGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACATCGGTCCCAAGCATCAAAGCTCGAGCTTTGATGCTTGGGACCGATG-3'

Other clones with same target sequence:

(none)