Construct: shRNA TRCN0000242908

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_152326.2-1382s21c1
DNA Barcode:
TGGGTGAGGACAAGTTCCAGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD9 (122416)
Gene Description:
ankyrin repeat domain 9
Transcript:
RefSeq NM_152326.2 (NON-CURRENT)
Match location:
Position 1382 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242908)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGGGTGAGGACAAGTTCCAGT
Hairpin Sequence:
5'-CCGG-TGGGTGAGGACAAGTTCCAGT-CTCGAG-ACTGGAACTTGTCCTCACCCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGGGTGAGGACAAGTTCCAGTCTCGAGACTGGAACTTGTCCTCACCCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGGGTGAGGACAAGTTCCAGTCTCGAGACTGGAACTTGTCCTCACCCA-3'

Other clones with same target sequence:

(none)