Construct: shRNA TRCN0000242938

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_182603.2-656s21c1
DNA Barcode:
TGCTTGTGTACAGGCTCTTAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ANKRD42 (338699)
Gene Description:
ankyrin repeat domain 42
Transcript:
RefSeq NM_182603.2 (NON-CURRENT)
Match location:
Position 656 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242938)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCTTGTGTACAGGCTCTTAT
Hairpin Sequence:
5'-CCGG-TGCTTGTGTACAGGCTCTTAT-CTCGAG-ATAAGAGCCTGTACACAAGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCTTGTGTACAGGCTCTTATCTCGAGATAAGAGCCTGTACACAAGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCTTGTGTACAGGCTCTTATCTCGAGATAAGAGCCTGTACACAAGCA-3'

Other clones with same target sequence:

(none)