Construct: shRNA TRCN0000242978

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_144992.4-2912s21c1
DNA Barcode:
ATGTCGTGTCTAAGGTCTTTG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
VWA3B (200403)
Gene Description:
von Willebrand factor A domain containing 3B
Transcript:
RefSeq NM_144992.4 (NON-CURRENT)
Match location:
Position 2912 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000242978)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ATGTCGTGTCTAAGGTCTTTG
Hairpin Sequence:
5'-CCGG-ATGTCGTGTCTAAGGTCTTTG-CTCGAG-CAAAGACCTTAGACACGACAT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGATGTCGTGTCTAAGGTCTTTGCTCGAGCAAAGACCTTAGACACGACATTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAATGTCGTGTCTAAGGTCTTTGCTCGAGCAAAGACCTTAGACACGACAT-3'

Other clones with same target sequence:

(none)