Construct: shRNA TRCN0000243057

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031477.4-1303s21c1
DNA Barcode:
CGGATCTAGCTCCTGTATATA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
YPEL3 (83719)
Gene Description:
yippee like 3
Transcript:
RefSeq NM_031477.4 (NON-CURRENT)
Match location:
Position 1303 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243057)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGGATCTAGCTCCTGTATATA
Hairpin Sequence:
5'-CCGG-CGGATCTAGCTCCTGTATATA-CTCGAG-TATATACAGGAGCTAGATCCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGGATCTAGCTCCTGTATATACTCGAGTATATACAGGAGCTAGATCCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGGATCTAGCTCCTGTATATACTCGAGTATATACAGGAGCTAGATCCG-3'

Other clones with same target sequence:

(none)