Construct: shRNA TRCN0000243124

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_001039211.2-1453s21c1
DNA Barcode:
TGCGCGACATCGCCATAATGA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ATAD3C (219293)
Gene Description:
ATPase family AAA domain containing 3C
Transcript:
RefSeq NM_001039211.2 (NON-CURRENT)
Match location:
Position 1453 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243124)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCGCGACATCGCCATAATGA
Hairpin Sequence:
5'-CCGG-TGCGCGACATCGCCATAATGA-CTCGAG-TCATTATGGCGATGTCGCGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCGCGACATCGCCATAATGACTCGAGTCATTATGGCGATGTCGCGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCGCGACATCGCCATAATGACTCGAGTCATTATGGCGATGTCGCGCA-3'

Other clones with same target sequence:

(none)