Construct: shRNA TRCN0000243189

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_005961.2-3262s21c1
DNA Barcode:
ACCTGCCACAGCAAGGTATAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
MUC6 (4588)
Gene Description:
mucin 6, oligomeric mucus/gel-forming
Transcript:
RefSeq NM_005961.2 (NON-CURRENT)
Match location:
Position 3262 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243189)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACCTGCCACAGCAAGGTATAC
Hairpin Sequence:
5'-CCGG-ACCTGCCACAGCAAGGTATAC-CTCGAG-GTATACCTTGCTGTGGCAGGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACCTGCCACAGCAAGGTATACCTCGAGGTATACCTTGCTGTGGCAGGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACCTGCCACAGCAAGGTATACCTCGAGGTATACCTTGCTGTGGCAGGT-3'

Other clones with same target sequence:

(none)