Construct: shRNA TRCN0000243212

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181610.1-194s21c1
DNA Barcode:
CCTACCCTGAGGACACGATAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
KRTAP19-4 (337971)
Gene Description:
keratin associated protein 19-4
Transcript:
RefSeq NM_181610.1 (NON-CURRENT)
Match location:
Position 194 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243212)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCTACCCTGAGGACACGATAT
Hairpin Sequence:
5'-CCGG-CCTACCCTGAGGACACGATAT-CTCGAG-ATATCGTGTCCTCAGGGTAGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCTACCCTGAGGACACGATATCTCGAGATATCGTGTCCTCAGGGTAGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCTACCCTGAGGACACGATATCTCGAGATATCGTGTCCTCAGGGTAGG-3'

Other clones with same target sequence:

(none)