Construct: shRNA TRCN0000243300

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033086.2-1110s21c1
DNA Barcode:
AGAAGTCATCATGGGCATATT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FGD3 (89846)
Gene Description:
FYVE, RhoGEF and PH domain containing 3
Transcript:
RefSeq NM_033086.2 (CURRENT)
Match location:
Position 1110 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243300)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGTCATCATGGGCATATT
Hairpin Sequence:
5'-CCGG-AGAAGTCATCATGGGCATATT-CTCGAG-AATATGCCCATGATGACTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGTCATCATGGGCATATTCTCGAGAATATGCCCATGATGACTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGTCATCATGGGCATATTCTCGAGAATATGCCCATGATGACTTCT-3'

Other clones with same target sequence:

(none)