Construct: shRNA TRCN0000243330

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033259.2-351s21c1
DNA Barcode:
TGATCGAGGATGACCGGATAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK2N2 (94032)
Gene Description:
calcium/calmodulin dependent protein kinase II inhibitor 2
Transcript:
RefSeq NM_033259.2 (NON-CURRENT)
Match location:
Position 351 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243330)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGATCGAGGATGACCGGATAG
Hairpin Sequence:
5'-CCGG-TGATCGAGGATGACCGGATAG-CTCGAG-CTATCCGGTCATCCTCGATCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGATCGAGGATGACCGGATAGCTCGAGCTATCCGGTCATCCTCGATCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGATCGAGGATGACCGGATAGCTCGAGCTATCCGGTCATCCTCGATCA-3'

Other clones with same target sequence:

  1. TRCN0000255331