Construct: shRNA TRCN0000243332

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_033259.2-1172s21c1
DNA Barcode:
CCAAGGGCCCAGATCCGATTT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
CAMK2N2 (94032)
Gene Description:
calcium/calmodulin dependent protein kinase II inhibitor 2
Transcript:
RefSeq NM_033259.2 (NON-CURRENT)
Match location:
Position 1172 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243332)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCAAGGGCCCAGATCCGATTT
Hairpin Sequence:
5'-CCGG-CCAAGGGCCCAGATCCGATTT-CTCGAG-AAATCGGATCTGGGCCCTTGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCAAGGGCCCAGATCCGATTTCTCGAGAAATCGGATCTGGGCCCTTGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCAAGGGCCCAGATCCGATTTCTCGAGAAATCGGATCTGGGCCCTTGG-3'

Other clones with same target sequence:

(none)