Construct: shRNA TRCN0000243366

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_024705.1-262s21c1
DNA Barcode:
CTTGTCTGATCCCAAGCAAAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
DHRS12 (79758)
Gene Description:
dehydrogenase/reductase 12
Transcript:
RefSeq NM_024705.1 (NON-CURRENT)
Match location:
Position 262 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243366)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CTTGTCTGATCCCAAGCAAAT
Hairpin Sequence:
5'-CCGG-CTTGTCTGATCCCAAGCAAAT-CTCGAG-ATTTGCTTGGGATCAGACAAG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCTTGTCTGATCCCAAGCAAATCTCGAGATTTGCTTGGGATCAGACAAGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACTTGTCTGATCCCAAGCAAATCTCGAGATTTGCTTGGGATCAGACAAG-3'

Other clones with same target sequence:

(none)