Construct: shRNA TRCN0000243431

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_150227.8-861s21c1
DNA Barcode:
AGAAGTTTGCTGTCCTAATTT

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Msl2 (77853)
Gene Description:
male-specific lethal 2 homolog (Drosophila)
Transcript:
RefSeq XM_150227.8 (NON-CURRENT)
Match location:
Position 861 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243431)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
AGAAGTTTGCTGTCCTAATTT
Hairpin Sequence:
5'-CCGG-AGAAGTTTGCTGTCCTAATTT-CTCGAG-AAATTAGGACAGCAAACTTCT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGAGAAGTTTGCTGTCCTAATTTCTCGAGAAATTAGGACAGCAAACTTCTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAAGAAGTTTGCTGTCCTAATTTCTCGAGAAATTAGGACAGCAAACTTCT-3'

Other clones with same target sequence:

(none)