Construct: shRNA TRCN0000243431
Construct Description:
- Construct Type:
- shRNA
- Other Identifiers:
- XM_150227.8-861s21c1
- DNA Barcode:
- AGAAGTTTGCTGTCCTAATTT
Original Target:
- Taxon:
-
Mus musculus (mouse)
- Gene:
- Msl2 (77853)
- Gene Description:
- male-specific lethal 2 homolog (Drosophila)
- Transcript:
- RefSeq XM_150227.8 (NON-CURRENT)
- Match location:
- Position 861 (CDS)
Vector Information:
- Vector Backbone:
- pLKO_005
- Pol II Cassette 1:
- PGK-PuroR
- Pol II Cassette 2:
- n/a
- Pol III Promoter:
- constitutive hU6
- Pol III Insert:
- (TRCN0000243431)
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
Current transcripts matched by this shRNA:
Sequence Information
- Target Sequence:
- AGAAGTTTGCTGTCCTAATTT
- Hairpin Sequence:
-
5'-CCGG-AGAAGTTTGCTGTCCTAATTT-CTCGAG-AAATTAGGACAGCAAACTTCT-TTTTTG-3'
Oligo design for arrayed cloning:
- Forward sequence:
- 5'-CCGGAGAAGTTTGCTGTCCTAATTTCTCGAGAAATTAGGACAGCAAACTTCTTTTTTG-3'
- Reverse sequence:
- 5'-AATTCAAAAAAGAAGTTTGCTGTCCTAATTTCTCGAGAAATTAGGACAGCAAACTTCT-3'
Other clones with same target sequence:
(none)
