Construct: shRNA TRCN0000243450

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030145.2-716s21c1
DNA Barcode:
ACTCACCATCCGTACCATTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Lsm6 (78651)
Gene Description:
LSM6 homolog, U6 small nuclear RNA and mRNA degradation associated
Transcript:
RefSeq NM_030145.2 (NON-CURRENT)
Match location:
Position 716 (3UTR)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243450)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
ACTCACCATCCGTACCATTTA
Hairpin Sequence:
5'-CCGG-ACTCACCATCCGTACCATTTA-CTCGAG-TAAATGGTACGGATGGTGAGT-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGACTCACCATCCGTACCATTTACTCGAGTAAATGGTACGGATGGTGAGTTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAAACTCACCATCCGTACCATTTACTCGAGTAAATGGTACGGATGGTGAGT-3'

Other clones with same target sequence:

(none)