Construct: shRNA TRCN0000243472

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_030248.1-1327s21c1
DNA Barcode:
TGCGGATGCAGCACCTGTTTA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Cdk5rap3 (80280)
Gene Description:
CDK5 regulatory subunit associated protein 3
Transcript:
RefSeq NM_030248.1 (NON-CURRENT)
Match location:
Position 1327 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243472)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
TGCGGATGCAGCACCTGTTTA
Hairpin Sequence:
5'-CCGG-TGCGGATGCAGCACCTGTTTA-CTCGAG-TAAACAGGTGCTGCATCCGCA-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGTGCGGATGCAGCACCTGTTTACTCGAGTAAACAGGTGCTGCATCCGCATTTTTG-3'
Reverse sequence:
5'-AATTCAAAAATGCGGATGCAGCACCTGTTTACTCGAGTAAACAGGTGCTGCATCCGCA-3'

Other clones with same target sequence:

(none)