Construct: shRNA TRCN0000243490

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_031999.2-475s21c1
DNA Barcode:
CGCTCATGAACTTGTACTTCA

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Gpr137b (83924)
Gene Description:
G protein-coupled receptor 137B
Transcript:
RefSeq NM_031999.2 (CURRENT)
Match location:
Position 475 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243490)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CGCTCATGAACTTGTACTTCA
Hairpin Sequence:
5'-CCGG-CGCTCATGAACTTGTACTTCA-CTCGAG-TGAAGTACAAGTTCATGAGCG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCGCTCATGAACTTGTACTTCACTCGAGTGAAGTACAAGTTCATGAGCGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACGCTCATGAACTTGTACTTCACTCGAGTGAAGTACAAGTTCATGAGCG-3'

Other clones with same target sequence:

(none)