Construct: shRNA TRCN0000243535

Construct Description:

Construct Type:
shRNA
Other Identifiers:
NM_181594.2-305s21c1
DNA Barcode:
CAACGAACAAACCTGGTATAC

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
Edc4 (234699)
Gene Description:
enhancer of mRNA decapping 4
Transcript:
RefSeq NM_181594.2 (NON-CURRENT)
Match location:
Position 305 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243535)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CAACGAACAAACCTGGTATAC
Hairpin Sequence:
5'-CCGG-CAACGAACAAACCTGGTATAC-CTCGAG-GTATACCAGGTTTGTTCGTTG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCAACGAACAAACCTGGTATACCTCGAGGTATACCAGGTTTGTTCGTTGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACAACGAACAAACCTGGTATACCTCGAGGTATACCAGGTTTGTTCGTTG-3'

Other clones with same target sequence:

(none)