Construct: shRNA TRCN0000243549

Construct Description:

Construct Type:
shRNA
Other Identifiers:
XM_982830.1-466s21c1
DNA Barcode:
CCCAGAAGAGTCTCTACAAAG

Original Target:

Taxon:
Mus musculus (mouse)
Gene:
LOC236260 (236260)
Gene Description:
hypothetical protein LOC236260
Transcript:
RefSeq XM_982830.1 (NON-CURRENT)
Match location:
Position 466 (CDS)

Vector Information:

Vector Backbone:
pLKO_005
Pol II Cassette 1:
PGK-PuroR
Pol II Cassette 2:
n/a
Pol III Promoter:
constitutive hU6
Pol III Insert:
(TRCN0000243549)
Selection Marker:
PuroR
Visible Reporter:
n/a

Current transcripts matched by this shRNA:

Sequence Information

Target Sequence:
CCCAGAAGAGTCTCTACAAAG
Hairpin Sequence:
5'-CCGG-CCCAGAAGAGTCTCTACAAAG-CTCGAG-CTTTGTAGAGACTCTTCTGGG-TTTTTG-3'

Oligo design for arrayed cloning:

Forward sequence:
5'-CCGGCCCAGAAGAGTCTCTACAAAGCTCGAGCTTTGTAGAGACTCTTCTGGGTTTTTG-3'
Reverse sequence:
5'-AATTCAAAAACCCAGAAGAGTCTCTACAAAGCTCGAGCTTTGTAGAGACTCTTCTGGG-3'

Other clones with same target sequence:

(none)